Download or read book New Techniques for Proteomics Study: Instrumentation, Separation, and Application written by Si Wu and published by . This book was released on 2006 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Second, to increase the mass measurement accuracy and protein identification confidence in FTICR-MS, we have developed a new calibration method, Calibration Optimization on Fragment Ions (COFI), for fragmentation data to correct the effects of space charge in FTICR-MS. There is a post-data processing strategy, and no need to include an internal calibrant or to apply ion population control. COFI has been successfully applied to different types of LC-FTICR fragmentation data, including LC-MS/MS data and multiplexed LC-CID data.